Park, Kyeong-Su Kim, Ju Shin, Hee Chung, Kyung-Sook Im, Dong-Soo Lim, Jung Jung, Cho-Rok Additional file 3: Figure S3. of E2-EPF UCP regulates stability and functions of missense mutant pVHL via ubiquitin mediated proteolysis Ubiquitination is decreased in lysine zero mutant pVHL in cells significantly. (C) Single-lysine (K159, K171, and K196) and lysine-zero mutant pVHLs were analyzed in vitro ubiquitination assay. GST-tagged VHL was incubated with E1, Flag-ubiquitin and His-UCP at 37 °C for 1 h. Each GST-VHL was pulled-down with glutathione sepharose beads and analyzed by anti-GST or anti-HA antibody. (TIFF 115 kb) VHL disease;pVHL missense mutation;E2-EPF UCP;Ubiquitination;Protein instability 2015-10-26
    https://springernature.figshare.com/articles/figure/Additional_file_3_Figure_S3_of_E2-EPF_UCP_regulates_stability_and_functions_of_missense_mutant_pVHL_via_ubiquitin_mediated_proteolysis/4397345
10.6084/m9.figshare.c.3622250_D1.v1