%0 Journal Article %A Lollo, Camila %A Vasconcelos, Dewton %A Oliveira, Luanda %A Titz, Tiago %A Carneiro-Sampaio, Magda %A Jacob, Cristina %A Duarte, Alberto %A Sato, Maria %D 2016 %T MOESM1 of Impaired CD8+ T cell responses upon Toll-like receptor activation in common variable immunodeficiency %U https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Impaired_CD8_T_cell_responses_upon_Toll-like_receptor_activation_in_common_variable_immunodeficiency/4397099 %R 10.6084/m9.figshare.c.3622163_D2.v1 %2 https://ndownloader.figshare.com/files/7123484 %K Common variable immunodeficiency %K Exhaustion and activation markers %K Toll-like receptor agonists %K Tc22/Th22 %K Polyfunctional T cells %X Additional file 1. Gating strategy for monofunctional CD4+ and CD8+ T cells. Initially, cells were selected from gates SSC-A and SSC-H (singlets) to exclude doublets; afterwards, we selected lymphocyte cells, live cells and CD3+ T cells, and the subsets CD4+ and CD8+ . Each cytokine was individually defined: IL-17a, IL-22, TNF, IL-10 and IFN-γ. %I figshare