%0 Journal Article %A Regna, Kimberly %A Kurshan, Peri %A Harwood, Benjamin %A Jenkins, Adam %A Lai, Chao-Qiang %A Muskavitch, Marc %A Kopin, Alan %A Draper, Isabelle %D 2016 %T Additional file 2: Figure S2. of A critical role for the Drosophila dopamine D1-like receptor Dop1R2 at the onset of metamorphosis %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_Figure_S2_of_A_critical_role_for_the_Drosophila_dopamine_D1-like_receptor_Dop1R2_at_the_onset_of_metamorphosis/4394384 %R 10.6084/m9.figshare.c.3621137_D5.v1 %2 https://ndownloader.figshare.com/files/7120748 %K Development %K Dopamine %K GPCR %K RNAi %K Transcriptome %X dsDop1R2 knockdown-induced lethality is recapitulated with alternate RNAi constructs. (A) and (B) Ubiquitous knockdown of Dop1R2. (A) VDRC 3391-GD (genotype: w1118;UAS-dsDop1R2/+;Act5C-Gal4/+) results in 98 % lethality at 29 °C (n = 58) and 94 % lethality at 25 °C, or viability (n = 44). (B) VDRC 105324-KK (genotype: w1118;TM6B/+;UAS-dsDop1R2/+) results in 97 % lethality at 29 °C (n = 59), compared to control balancer siblings (genotypes: w1118;CyO/+;UAS-dsDop1R2/+ and w1118;CyO/UAS-dsDop1R2, respectively). All male escaper flies (n = 4) obtained when using the VDRC 3391-GD RNAi construct exhibited the hypomelanization phenotype (described in Fig. 7). (C) Salivary gland/amnioserosa targeted knockdown of Dop1R2 VDRC 3391-GD results in 68 % lethality at 29 °C in experimental flies (genotype: w1118;UAS-dsDop1R2/+;P{GawB}c729-GAL4/+), compared to controls (genotype: w1118;P{GawB}c729-Gal4/+) (n = 53). VDRC Dop1R2 knockdown stocks: 3391-GD (FBst0460369) and 105324-KK (FBst0477151). Driver stocks: Act5C-GAL4 (FBst0003954), P{GawB}17A-GAL4 (FBst0008474) and P{GawB}c729-GAL4 (FBst0006983). (PDF 230 kb) %I figshare