TY - DATA T1 - Additional file 6: of Comprehensive profiling of functional Epstein-Barr virus miRNA expression in human cell lines PY - 2016/08/17 AU - Marjolein Hooykaas AU - Elisabeth Kruse AU - Emmanuel Wiertz AU - Robert Lebbink UR - https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_of_Comprehensive_profiling_of_functional_Epstein-Barr_virus_miRNA_expression_in_human_cell_lines/4376762 DO - 10.6084/m9.figshare.c.3615557_D1.v1 L4 - https://ndownloader.figshare.com/files/7102889 KW - Epstein-Barr virus (EBV) KW - Herpesvirus KW - microRNAs KW - miRNA reporter KW - miRNA sensor KW - Small RNA sequencing N2 - AKBM cells are EBV positive. A) Schematic representation of the EBV super-sensor. Five perfect EBV miRNA target sites were cloned downstream of the mCherry reporter gene. The vector allows for sensing of EBV presence by monitoring the combined functional expression of 5 EBV miRNAs. B) EBV-positive AKBM cells and EBV negative AK31 and 2A8 cells were either left untreated (cells alone), were transduced with an mCherry-control vector (control sensor), or were transduced with the EBV sensor from a). Cells were monitored for mCherry reporter expression at 7 days post transduction by flow cytometry (PDF 165 kb) ER -