%0 Figure %A Asperti, Claudia %A Astro, Veronica %A Pettinato, Emanuela %A Paris, Simona %A Bachi, Angela %A de Curtis, Ivan %D 2011 %T Expression of GIT1-C affects cell morphology and the distribution of endogenous liprin-α1. %U https://plos.figshare.com/articles/figure/_Expression_of_GIT1_C_affects_cell_morphology_and_the_distribution_of_endogenous_liprin_945_1_/436819 %R 10.1371/journal.pone.0020757.g004 %2 https://ndownloader.figshare.com/files/766453 %K git1-c %K affects %K morphology %K endogenous %X

(A) COS7 cells transfected for one day with either FLAG-GIT1, FLAG-GIT1-C, or FLAG-βGalactosidase were re-plated for 1 h on FN. Immunofluorescence for the transfected proteins (FLAG), paxillin, and phalloidin staining for F-actin. Scale bar, 20 µm. Below, 3-fold enlargements of areas from cells stained for paxillin (arrowheads in the corresponding cells above) are shown. (B) Expression of GIT1-C induces a significant increase of cell spreading on FN. Bars are means ± SEM (n = 116–121 cells per condition); *P<0.05. (C) Cells transfected with either FLAG-βGalactosidase or FLAG-GIT1-C were used for triple immunofluorescence staining with antibodies for endogenous liprin-α1, paxillin, and transfected proteins (FLAG): endogenous liprin-α1 accumulates at the edge of GIT1-C-transfected cells. Scale bar, 5 µm.

%I PLOS ONE