Proportions of sporocyte and mature cyst stages of <i>Pneumocystis carinii</i>. Anna Martinez El Moukhtar Aliouat Annie Standaert-Vitse Elisabeth Werkmeister Muriel Pottier Claire Pinçon Eduardo Dei-Cas Cécile-Marie Aliouat-Denis 10.1371/journal.pone.0020935.t002 https://plos.figshare.com/articles/dataset/_Proportions_of_sporocyte_and_mature_cyst_stages_of_Pneumocystis_carinii_/436807 <p>Whole organism population was isolated from the lungs of immunosuppressed animals as previously described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020935#pone.0020935-Aviles1" target="_blank">[27]</a>. Sporocyte and mature cyst stages were counted from RAL-555-stained smears according to the number of nuclei in each life cycle stage. Percentages of sporocyte and cystic stages with N nuclei in the fraction of sporocytes and mature cysts were given as mean of three independent experiments (n = 70, n = 55, n = 50). N = number of nuclei per fungal cell. SD = standard deviation.</p>(a)<p>Early sporocytes.</p>(b)<p>Sum of intermediate sporocytes with 2, 3 and 4 nuclei.</p>(c)<p>Sum of late sporocytes and mature cysts with 5, 6, 7 and 8 nuclei.</p> 2013-02-20 17:38:01 sporocyte mature cyst stages