%0 Figure %A Chen, Aaron Yun %A Kleiboeker, Steve %A Qiu, Jianming %D 2011 %T Support of B19V infection of CD36+ EPCs under hypoxia vs. normoxia. %U https://plos.figshare.com/articles/figure/_Support_of_B19V_infection_of_CD36_EPCs_under_hypoxia_vs_normoxia_/435543 %R 10.1371/journal.ppat.1002088.g001 %2 https://ndownloader.figshare.com/files/765166 %K b19v %K epcs %K hypoxia %X

(A) Status of CD36+ EPCs cultured under hypoxia vs. normoxia. Day 4 HSCs were ex vivo expanded under either hypoxia or normoxia to produce CD36+ EPCs. At day 8, the cells were profiled by flow cytometry analysis for surface antigens indicated. Numbers shown in each plot are percentage of positive cells. (B&C) B19V infection under hypoxia vs. normoxia. Day 8 CD36+ EPCs were infected with B19V at an MOI of 5,000 gc/cell. (B) At 48 hrs p.i., immunofluorescence staining was performed using anti-NS1 or anti-11 kDa antiserum. DAPI was used to stain the nucleus. Confocal images were taken at a magnification of 40×(objective lens) using an Eclipse C1 Plus confocal microscope controlled by EZ-C1 software (Nikon). (C) At 48 hrs p.i., mRNA was extracted from infected cells, and the levels of B19V VP2-encoding mRNA per β-actin mRNA were quantified.

%I PLOS Pathogens