10.6084/m9.figshare.c.3606494_D5.v1
Dimitris Kyriakou
Dimitris
Kyriakou
Emmanouil Stavrou
Emmanouil
Stavrou
Panayiota Demosthenous
Panayiota
Demosthenous
Georgia Angelidou
Georgia
Angelidou
Bryan-Joseph San Luis
Bryan-Joseph
San Luis
Charles Boone
Charles
Boone
Vasilis Promponas
Vasilis
Promponas
Antonis Kirmizis
Antonis
Kirmizis
Additional file 10: Figure S5. of Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae
Springer Nature
2016
Long intergenic non-coding RNAs
Stable unannotated transcripts
Synthetic genetic array
Genetic interactions
Telomere
2016-12-07 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_Figure_S5_of_Functional_characterisation_of_long_intergenic_non-coding_RNAs_through_genetic_interaction_profiling_in_Saccharomyces_cerevisiae/4348787
Degradation of telomeric ssDNA by bacterial Exonuclease I. Genomic DNA extracted from four individual sut457Δ strains at passage five was either untreated (-) or digested with bacterial exonuclease I (+), which degrades telomeric overhang DNA in the 3’ to 5’ direction, before being subjected to native southern analysis using a biotinylated probe against telomeric repeats (upper blot). The DNA on the membrane was then denatured with 0.4 N NaOH and re-probed to monitor equal loading (lower blot). (PDF 48 kb)