10.6084/m9.figshare.c.3606494_D5.v1 Dimitris Kyriakou Dimitris Kyriakou Emmanouil Stavrou Emmanouil Stavrou Panayiota Demosthenous Panayiota Demosthenous Georgia Angelidou Georgia Angelidou Bryan-Joseph San Luis Bryan-Joseph San Luis Charles Boone Charles Boone Vasilis Promponas Vasilis Promponas Antonis Kirmizis Antonis Kirmizis Additional file 10: Figure S5. of Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae Springer Nature 2016 Long intergenic non-coding RNAs Stable unannotated transcripts Synthetic genetic array Genetic interactions Telomere 2016-12-07 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_Figure_S5_of_Functional_characterisation_of_long_intergenic_non-coding_RNAs_through_genetic_interaction_profiling_in_Saccharomyces_cerevisiae/4348787 Degradation of telomeric ssDNA by bacterial Exonuclease I. Genomic DNA extracted from four individual sut457Δ strains at passage five was either untreated (-) or digested with bacterial exonuclease I (+), which degrades telomeric overhang DNA in the 3’ to 5’ direction, before being subjected to native southern analysis using a biotinylated probe against telomeric repeats (upper blot). The DNA on the membrane was then denatured with 0.4 N NaOH and re-probed to monitor equal loading (lower blot). (PDF 48 kb)