10.6084/m9.figshare.c.3603338_D13.v1 Bethan Lloyd-Lewis Bethan Lloyd-Lewis Felicity Davis Felicity Davis Olivia Harris Olivia Harris Jessica Hitchcock Jessica Hitchcock Filipe Lourenco Filipe Lourenco Mathias Pasche Mathias Pasche Christine Watson Christine Watson Additional file 2: Figure S2. of Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods Springer Nature 2016 Mammary gland Lactation Breast cancer Tissue clearing 3D imaging Fluorescence microscopy Light sheet fluorescence microscopy Two-photon microscopy 2016-12-13 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_Figure_S2_of_Imaging_the_mammary_gland_and_mammary_tumours_in_3D_optical_tissue_clearing_and_immunofluorescence_methods/4339688 3DISCO clearing and 3D imaging of virgin and lactating mouse mammary tissue. a 3DISCO tissue clearing and immunostaining protocol and timeline. b Transmission images of 3DISCO cleared tissue. c Volume changes caused by 3DISCO-based clearing of virgin and lactating mammary tissue. Values are representative of measurements from three tissue pieces at each developmental timepoint. d 3D confocal imaging of 3DISCO-cleared virgin and lactating mammary glands immunostained with the basal cellb marker SMA. These images are representative of images from more than two mice. See Additional file 18 for a high resolution version of these PDFs. (PDF 8 mb)