10.6084/m9.figshare.c.3603338_D9.v1 Bethan Lloyd-Lewis Bethan Lloyd-Lewis Felicity Davis Felicity Davis Olivia Harris Olivia Harris Jessica Hitchcock Jessica Hitchcock Filipe Lourenco Filipe Lourenco Mathias Pasche Mathias Pasche Christine Watson Christine Watson Additional file 9: Figure S8. of Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods Springer Nature 2016 Mammary gland Lactation Breast cancer Tissue clearing 3D imaging Fluorescence microscopy Light sheet fluorescence microscopy Two-photon microscopy 2016-12-13 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_9_Figure_S8_of_Imaging_the_mammary_gland_and_mammary_tumours_in_3D_optical_tissue_clearing_and_immunofluorescence_methods/4339589 Three-dimensional confocal images of mammary glands cleared using a modified CUBIC clearing protocol (Reagent 1A) to minimise protein loss. Images show the maximum intensity projection of the entire image sequence. Bottom right panel shows depth-coding of SMA-expressing myoepithelial cells in lactating mammary tissue; images in an image stack are assigned a colour based on their relative depth. Arrowhead shows non-specific intraluminal staining occasionally observed with modified CUBIC clearing, which may be improved with further washing. These images are representative of images from two mice. BV blood vessel (SMA-expressing). See Additional file 18 for a high resolution version of these PDFs. (PDF 12 mb)