10.6084/m9.figshare.c.3603338_D9.v1
Bethan Lloyd-Lewis
Bethan
Lloyd-Lewis
Felicity Davis
Felicity
Davis
Olivia Harris
Olivia
Harris
Jessica Hitchcock
Jessica
Hitchcock
Filipe Lourenco
Filipe
Lourenco
Mathias Pasche
Mathias
Pasche
Christine Watson
Christine
Watson
Additional file 9: Figure S8. of Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods
Springer Nature
2016
Mammary gland
Lactation
Breast cancer
Tissue clearing
3D imaging
Fluorescence microscopy
Light sheet fluorescence microscopy
Two-photon microscopy
2016-12-13 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_9_Figure_S8_of_Imaging_the_mammary_gland_and_mammary_tumours_in_3D_optical_tissue_clearing_and_immunofluorescence_methods/4339589
Three-dimensional confocal images of mammary glands cleared using a modified CUBIC clearing protocol (Reagent 1A) to minimise protein loss. Images show the maximum intensity projection of the entire image sequence. Bottom right panel shows depth-coding of SMA-expressing myoepithelial cells in lactating mammary tissue; images in an image stack are assigned a colour based on their relative depth. Arrowhead shows non-specific intraluminal staining occasionally observed with modified CUBIC clearing, which may be improved with further washing. These images are representative of images from two mice. BV blood vessel (SMA-expressing). See Additional file 18 for a high resolution version of these PDFs. (PDF 12 mb)