Supplementary Material for: Calcium-Phosphate-Osteopontin Particles Reduce Biofilm Formation and pH Drops in in situ Grown Dental Biofilms
Schlafer S.
Ibsen C.J.S.
Birkedal H.
Nyvad B.
10.6084/m9.figshare.4312307.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Calcium-Phosphate-Osteopontin_Particles_Reduce_Biofilm_Formation_and_pH_Drops_in_in_situ_Grown_Dental_Biofilms/4312307
<p>This 2-period crossover study investigated the effect of
calcium-phosphate-osteopontin particles on biofilm formation and pH in
48-h biofilms grown in situ. Bovine milk osteopontin is a highly
phosphorylated glycoprotein that has been shown to interfere with
bacterial adhesion to salivary-coated surfaces.
Calcium-phosphate-osteopontin particles have been shown to reduce
biofilm formation and pH drops in a 5-species laboratory model of dental
biofilm without affecting bacterial viability. Here, smooth surface
biofilms from 10 individuals were treated ex vivo 6 times/day for 30 min
with either calcium-phosphate-osteopontin particles or sterile saline.
After growth, the amount of biofilm formed was determined by confocal
microscopy, and pH drops upon exposure to glucose were monitored using
confocal-microscopy-based pH ratiometry. A total of 160 biofilms were
analysed. No adverse effects of repeated ex vivo treatment with
calcium-phosphate-osteopontin particles were observed. Particle
treatment resulted in a 32% lower amount of biofilm formed (<i>p</i>
< 0.05), but large inter-individual differences could be observed.
Biofilm pH was significantly higher upon particle treatment, both
shortly after the addition of glucose and after 30 min of incubation
with glucose (<i>p</i> < 0.05). Calcium-phosphate-osteopontin
particles may represent a new therapeutic approach to caries control and
aim at directly targeting virulence factors involved in the caries
process. Further studies are required to determine the effect of
particle treatment on more acidogenic/aciduric biofilms as well as the
remineralizing potential of the particles.</p>
2016-12-13 14:54:48
Biofilms
Calcium
Dental caries
Osteopontin
Phosphorus