Tomás, Gonzalo Hernández, Martín Marandino, Ana Techera, Claudia Grecco, Sofia Hernández, Diego Banda, Alejandro Panzera, Yanina Pérez, Ruben Development of an RT-qPCR assay for the specific detection of a distinct genetic lineage of the infectious bursal disease virus <p>The infectious bursal disease virus (IBDV) is a major health threat to the world’s poultry industry despite intensive controls including proper biosafety practices and vaccination. IBDV (<i>Avibirnavirus, Birnaviridae</i>) is a non-enveloped virus with a bisegmented double-stranded RNA genome. The virus is traditionally classified into classic, variant and very virulent strains, each with different epidemiological relevance and clinical implications. Recently, a novel worldwide spread genetic lineage was described and denoted as distinct (d) IBDV. Here, we report the development and validation of a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay for the specific detection of dIBDVs in the global poultry industry. The assay employs a TaqMan-MGB probe that hybridizes with a unique molecular signature of dIBDV. The assay successfully detected all the assessed strains belonging to the dIBDV genetic lineage, showing high specificity and absence of cross-reactivity with non-dIBDVs, IBDV-negative samples and other common avian viruses. Using serial dilutions of <i>in vitro</i>-transcribed RNA we obtained acceptable PCR efficiencies and determination coefficients, and relatively small intra- and inter-assay variability. The assay demonstrated a wide dynamic range between 10<sup>3</sup> and 10<sup>8</sup> RNA copies/reaction. This rapid, specific and quantitative assay is expected to improve IBDV surveillance and control worldwide and to increase our understanding of the molecular epidemiology of this economically detrimental poultry pathogen.</p> IBDV;RT-qPCR;TaqMan-MGB probe;distinct IBDV;IBD 2016-12-07
    https://tandf.figshare.com/articles/dataset/Development_of_an_RT-qPCR_assay_for_the_specific_detection_of_a_distinct_genetic_lineage_of_the_infectious_bursal_disease_virus/4290758
10.6084/m9.figshare.4290758.v1