%0 Figure %A G. Cooper, Kendal %A Winfree, Seth %A Malik-Kale, Preeti %A Jolly, Carrie %A Ireland, Robin %A A. Knodler, Leigh %A Steele-Mortimer, Olivia %D 2011 %T Akt regulators accumulate in Salmonella induced ruffles. %U https://plos.figshare.com/articles/figure/_Akt_regulators_accumulate_in_Salmonella_induced_ruffles_/427587 %R 10.1371/journal.pone.0022260.g005 %2 https://ndownloader.figshare.com/files/757220 %K regulators %K accumulate %K induced %X

HeLa cells were transfected with plasmids encoding epitope-tagged proteins as indicated. After 20 hr they were infected with Salmonella for 30 min, then fixed and processed for immunofluorescence microscopy. GFP-HA-Akt transfected cells were stained for Salmonella LPS (Cy5) and phospho Akt Ser473 (AF568: A–C), Myc (AF568: D–F) or 3×FLAG (AF568: J–L). Cells expressing PDK1-Myc (G–I) were stained for LPS (Cy5), Myc (AF488) and actin filaments (phalloidin-AF568) to reveal the ruffles.

%I PLOS ONE