Akt regulators accumulate in <i>Salmonella</i> induced ruffles.
Kendal G. Cooper
Seth Winfree
Preeti Malik-Kale
Carrie Jolly
Robin Ireland
Leigh A. Knodler
Olivia Steele-Mortimer
10.1371/journal.pone.0022260.g005
https://plos.figshare.com/articles/figure/_Akt_regulators_accumulate_in_Salmonella_induced_ruffles_/427587
<p>HeLa cells were transfected with plasmids encoding epitope-tagged proteins as indicated. After 20 hr they were infected with <i>Salmonella</i> for 30 min, then fixed and processed for immunofluorescence microscopy. GFP-HA-Akt transfected cells were stained for <i>Salmonella</i> LPS (Cy5) and phospho Akt Ser473 (AF568: A–C), Myc (AF568: D–F) or 3×FLAG (AF568: J–L). Cells expressing PDK1-Myc (G–I) were stained for LPS (Cy5), Myc (AF488) and actin filaments (phalloidin-AF568) to reveal the ruffles.</p>
2011-07-14 02:06:27
regulators
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