10.1371/journal.pgen.1006459.g001
Laura Fontenas
Laura
Fontenas
Flavia De Santis
Flavia
De Santis
Vincenzo Di Donato
Vincenzo
Di Donato
Cindy Degerny
Cindy
Degerny
Béatrice Chambraud
Béatrice
Chambraud
Filippo Del Bene
Filippo
Del Bene
Marcel Tawk
Marcel
Tawk
Characterization of the ndrg4 mutant.
Public Library of Science
2016
axon
analysis
ion channels
Zebrafish Axon ensheathment
ndrg 4 mutants
Ranvier
nod
vesicle
ndrg 4 functions
expression
Neuronal Ndrg 4
sodium channel
ndrg 4
2016-11-30 17:44:02
Figure
https://plos.figshare.com/articles/figure/Characterization_of_the_ndrg4_mutant_/4275233
<p>(A) Schematic representation of the <i>ndrg4</i> genomic locus. The extended region on the <i>exon 2</i> represents the sequence targeted by the CRISPR/Cas9 system. Red: sgRNA binding site. Blue: PAM sequence. <i>ndrg4</i><sup><i>+</i></sup> corresponds to the wild-type allele; <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> are the loss-of-function alleles used in this study. (B) Schematic of ndrg4 protein product. In <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> mutant fish, the deletions result in a frameshift generating a premature STOP codon at the level of the amino acids 31 and 34 (of 352) in the ndr family domain. Lateral views of a control (C) and a ndrg4 mutant (D) embryos at 72 hpf. The arrows point to the heart, note the pronounced heart edema (white asterisk) observed in the ndrg4 mutant. Lateral view of <i>ndrg4</i> mRNA expression in a control (E) and a ndrg4-/- embryo (F) at 48hpf. Note the absence of <i>ndrg4</i> expression in the mutant. Scale bar = 200 μm.</p>