10.1371/journal.pgen.1006459.g001 Laura Fontenas Laura Fontenas Flavia De Santis Flavia De Santis Vincenzo Di Donato Vincenzo Di Donato Cindy Degerny Cindy Degerny Béatrice Chambraud Béatrice Chambraud Filippo Del Bene Filippo Del Bene Marcel Tawk Marcel Tawk Characterization of the ndrg4 mutant. Public Library of Science 2016 axon analysis ion channels Zebrafish Axon ensheathment ndrg 4 mutants Ranvier nod vesicle ndrg 4 functions expression Neuronal Ndrg 4 sodium channel ndrg 4 2016-11-30 17:44:02 Figure https://plos.figshare.com/articles/figure/Characterization_of_the_ndrg4_mutant_/4275233 <p>(A) Schematic representation of the <i>ndrg4</i> genomic locus. The extended region on the <i>exon 2</i> represents the sequence targeted by the CRISPR/Cas9 system. Red: sgRNA binding site. Blue: PAM sequence. <i>ndrg4</i><sup><i>+</i></sup> corresponds to the wild-type allele; <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> are the loss-of-function alleles used in this study. (B) Schematic of ndrg4 protein product. In <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> mutant fish, the deletions result in a frameshift generating a premature STOP codon at the level of the amino acids 31 and 34 (of 352) in the ndr family domain. Lateral views of a control (C) and a ndrg4 mutant (D) embryos at 72 hpf. The arrows point to the heart, note the pronounced heart edema (white asterisk) observed in the ndrg4 mutant. Lateral view of <i>ndrg4</i> mRNA expression in a control (E) and a ndrg4-/- embryo (F) at 48hpf. Note the absence of <i>ndrg4</i> expression in the mutant. Scale bar = 200 μm.</p>