Temporal transcriptome of IMR90 fibroblasts inducibly expressing MCPyV ST. Christian Berrios Megha Padi Mark A. Keibler Donglim Esther Park Vadim Molla Jingwei Cheng Soo Mi Lee Gregory Stephanopoulos John Quackenbush James A. DeCaprio 10.1371/journal.ppat.1006020.g001 https://plos.figshare.com/articles/figure/Temporal_transcriptome_of_IMR90_fibroblasts_inducibly_expressing_MCPyV_ST_/4252334 <p><b>A)</b> IMR90 fibroblasts containing dox-inducible MCPyV ST or GFP vectors were treated with dox and harvested every 8 hours for RNA extraction. Each time point represents three biological replicas. <b>B)</b> Mean ST transcript levels and <b>C)</b> immunoblotting for ST, GFP and vinculin from cells collected every 8 hours for 96 hours following dox treatment. <b>D)</b> Hierarchical clustering and fold change between MCPyV ST and GFP following dox induction for 96 hours. Each bar represents an average of three experiments for each time point. The enrichment of “Cancer Hallmark” gene sets are represented relative to the ST-differentially expressed clusters, including epithelial to mesenchymal transition (EMT), tumor necrosis factor-α (TNFA signaling via NF-κB), hypoxia, mTORC1, oxidative phosphorylation, glycolysis, MYC, and several cell cycle clusters including E2F targets, G2M checkpoint and mitotic spindle. The color bar indicates statistical significance, yellow p < 0.05 and gray p > 0.05.</p> 2016-11-23 04:30:02 T Antigen Promotes Pro-Glycolytic Metabolic Perturbations MCPyV-induced MCT 1 levels MYC transcriptome Merkel cell carcinoma MCC cell lines MCT 1 induction MYCN MYCL MCT 1 levels Transformation Merkel cell polyomavirus MCPyV ST NF Several MCC lines IMR 90 cells MCT 1 expression monocarboxylate lactate transporter SLC 16A Merkel Cell Polyomavirus MCT 1 activity