Temporal transcriptome of IMR90 fibroblasts inducibly expressing MCPyV ST.
Christian Berrios
Megha Padi
Mark A. Keibler
Donglim Esther Park
Vadim Molla
Jingwei Cheng
Soo Mi Lee
Gregory Stephanopoulos
John Quackenbush
James A. DeCaprio
10.1371/journal.ppat.1006020.g001
https://plos.figshare.com/articles/figure/Temporal_transcriptome_of_IMR90_fibroblasts_inducibly_expressing_MCPyV_ST_/4252334
<p><b>A)</b> IMR90 fibroblasts containing dox-inducible MCPyV ST or GFP vectors were treated with dox and harvested every 8 hours for RNA extraction. Each time point represents three biological replicas. <b>B)</b> Mean ST transcript levels and <b>C)</b> immunoblotting for ST, GFP and vinculin from cells collected every 8 hours for 96 hours following dox treatment. <b>D)</b> Hierarchical clustering and fold change between MCPyV ST and GFP following dox induction for 96 hours. Each bar represents an average of three experiments for each time point. The enrichment of “Cancer Hallmark” gene sets are represented relative to the ST-differentially expressed clusters, including epithelial to mesenchymal transition (EMT), tumor necrosis factor-α (TNFA signaling via NF-κB), hypoxia, mTORC1, oxidative phosphorylation, glycolysis, MYC, and several cell cycle clusters including E2F targets, G2M checkpoint and mitotic spindle. The color bar indicates statistical significance, yellow p < 0.05 and gray p > 0.05.</p>
2016-11-23 04:30:02
T Antigen Promotes Pro-Glycolytic Metabolic Perturbations
MCPyV-induced MCT 1 levels
MYC
transcriptome
Merkel cell carcinoma
MCC cell lines
MCT 1 induction
MYCN
MYCL
MCT 1 levels
Transformation Merkel cell polyomavirus
MCPyV ST
NF
Several MCC lines
IMR 90 cells
MCT 1 expression
monocarboxylate lactate transporter SLC 16A
Merkel Cell Polyomavirus
MCT 1 activity