%0 Generic %A O.Z., Ameer %A M., Butlin %A E., Kaschina %A M., Sommerfeld %A A.P., Avolio %A J.K., Phillips %D 2016 %T Supplementary Material for: Long-Term Angiotensin II Receptor Blockade Limits Hypertension, Aortic Dysfunction, and Structural Remodeling in a Rat Model of Chronic Kidney Disease %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Long-Term_Angiotensin_II_Receptor_Blockade_Limits_Hypertension_Aortic_Dysfunction_and_Structural_Remodeling_in_a_Rat_Model_of_Chronic_Kidney_Disease/4249907 %R 10.6084/m9.figshare.4249907.v1 %2 https://ndownloader.figshare.com/files/6929381 %2 https://ndownloader.figshare.com/files/6929384 %2 https://ndownloader.figshare.com/files/6929390 %K Valsartan %K Chronic kidney disease %K Aorta %K Vascular remodeling %K Endothelium %K Nitric oxide %K Calcification %X

Background/Aims: Chronic kidney disease (CKD) is associated with large artery remodeling, endothelial dysfunction and calcification, with angiotensin II (Ang II) a known driver of these pathologies. We investigated long-term Ang II type 1 receptor inhibition with valsartan on aortic function and structure in the Lewis polycystic kidney (LPK) rat model of CKD. Methods: Mixed sex LPK and Lewis control (total n = 28) treated (valsartan 60 mg/kg/day p.o. from 4 to 18 weeks) and vehicle groups were studied. Functional responses to noradrenaline (NA), potassium chloride and endothelium-dependent and independent relaxations were investigated in vitro using acetylcholine hydrochloride (ACh) and sodium nitroprusside (SNP), respectively. Effects of the nitric oxide synthase (NOS) substrate L-arginine, NOS inhibitor L-NAME and cyclooxygenase inhibitor indomethacin on ACh responses were examined. Results: In the LPK, valsartan reduced systolic blood pressure and urinary protein, ameliorated exaggerated sensitivity to NA, and normalized endothelium-dependent (ACh-Rmax; 91 ± 7 vs. 59 ± 6%, p = 0.0001) and independent dysfunction (SNP-Rmax; 99 ± 1 vs. 82 ± 7%, p = 0.040), as well as improving NO-dependent relaxation (Rmax; -51 ± 6 vs. -26 ± 9%, p = 0.008). Valsartan also reduced aortic wall hypertrophy, elastin disruption/fragmentation, calcification, media cystic degeneration, and levels of matrix metalloproteinase 9. Conclusions: This study highlights the role of Ang II in driving vascular manifestations of CKD and indicates that early treatment can significantly limit pathological changes.

%I Karger Publishers