TY - DATA T1 - EPR Imaging Spin Probe Trityl Radical OX063: A Method for Its Isolation from Animal Effluent, Redox Chemistry of Its Quinone Methide Oxidation Product, and in Vivo Application in a Mouse PY - 2016/11/07 AU - Maciej Serda AU - Yen-Ku Wu AU - Eugene D. Barth AU - Howard J. Halpern AU - Viresh H. Rawal UR - https://acs.figshare.com/articles/journal_contribution/EPR_Imaging_Spin_Probe_Trityl_Radical_OX063_A_Method_for_Its_Isolation_from_Animal_Effluent_Redox_Chemistry_of_Its_Quinone_Methide_Oxidation_Product_and_in_Vivo_Application_in_a_Mouse/4246703 DO - 10.1021/acs.chemrestox.6b00277.s001 L4 - https://ndownloader.figshare.com/files/6925013 KW - Vivo Application KW - probe KW - Redox Chemistry KW - Animal Effluent KW - Quinone Methide Oxidation Product KW - recovery KW - QM KW - crude lyophilizate KW - animal model EPR imaging experiments KW - quinone methide N2 - We report herein a method for the recovery, purification, and application of OX063, a costly, commercially available nontoxic spin probe widely used for electron paramagnetic resonance (EPR) imaging, as well as its corresponding quinone methide (QM) form. This precious probe can be successfully recovered after use in animal model experiments (25–47% recovery from crude lyophilizate with 98.5% purity), even from samples that are >2 years old. Significantly, the recovered trityl can be reused in further animal model EPR imaging experiments. The work also describes support for the observed formation of an air-sensitive radical derived from the QM under reducing conditions. ER -