%0 Figure %A Oliaro-Bosso, Simonetta %A Caron, Giulia %A Taramino, Silvia %A Ermondi, Giuseppe %A Viola, Franca %A Balliano, Gianni %D 2011 %T Residual activity of mutants C457D, C457D/A525F, C457D/N211D, C457D/N211A and C457D/N211K, after preincubation at 35°C. %U https://plos.figshare.com/articles/figure/_Residual_activity_of_mutants_C457D_C457D_A525F_C457D_N211D_C457D_N211A_and_C457D_N211K_after_preincubation_at_35_176_C_/424543 %R 10.1371/journal.pone.0022134.g004 %2 https://ndownloader.figshare.com/files/754173 %K mutants %K preincubation %X

Residual enzymatic activity per mg of protein of mutants C457D (♦), C457D/A525F (▪), C457D/N211D (▴), C457D/N211A (▵) and C457D/N211K (•) was determined after 30, 60, 120 and 240 min of preincubation at 35°C in the absence of substrate. Dotted lines are used for the mutants at position 211. After preincubation, the homogenates were incubated with substrate for 4 h at 25°C. Values are the means of at list two separate experiments, each one carried out in duplicate.

%I PLOS ONE