Residual activity of mutants C457D, C457D/A525F, C457D/N211D, C457D/N211A and C457D/N211K, after preincubation at 35°C. Simonetta Oliaro-Bosso Giulia Caron Silvia Taramino Giuseppe Ermondi Franca Viola Gianni Balliano 10.1371/journal.pone.0022134.g004 https://plos.figshare.com/articles/figure/_Residual_activity_of_mutants_C457D_C457D_A525F_C457D_N211D_C457D_N211A_and_C457D_N211K_after_preincubation_at_35_176_C_/424543 <p>Residual enzymatic activity per mg of protein of mutants C457D (♦), C457D/A525F (▪), C457D/N211D (▴), C457D/N211A (▵) and C457D/N211K (•) was determined after 30, 60, 120 and 240 min of preincubation at 35°C in the absence of substrate. Dotted lines are used for the mutants at position 211. After preincubation, the homogenates were incubated with substrate for 4 h at 25°C. Values are the means of at list two separate experiments, each one carried out in duplicate.</p> 2011-07-21 01:15:43 mutants preincubation