<i>Digitor</i> mutant third instar larvae have a severely reduced brain size. Saheli Sengupta Uttama Rath Changfu Yao Michael Zavortink Chao Wang Jack Girton Kristen M. Johansen Jørgen Johansen 10.1371/journal.pone.0166829.g008 https://plos.figshare.com/articles/figure/_i_Digitor_i_mutant_third_instar_larvae_have_a_severely_reduced_brain_size_/4243979 <p>(A) Images of dissected brains from wild type (WT) and <i>Digitor</i> mutant (<i>EP(3)3709/EP(3)3709</i>) third instar larvae at the same scale. (B) Comparison of the average area of the two optic lobes from 20 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference in size was statistically significant (P value < 0.001, Student's two-tailed t-test). (C) Images of wild type and <i>Digitor</i> mutant third instar larvae side by side. (D) Comparison of the average from four determinations of the body weight of 22 wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference in weight was not statistically significant (P value ≥ 0.8, Student's two-tailed t-test). (E) Acridine Orange labeling of brains from wild type and <i>Digitor</i> mutant larvae. (F) TUNEL labeling of brains from wild type and <i>Digitor</i> mutant larvae. (G) BrdU incorporation into brains from wild type and <i>Digitor</i> mutant larvae. (H) Anti-H3S10ph antibody labeling of brains from wild type and <i>Digitor</i> mutant larvae. (I) Comparison of the average fluorescence intensity of BrdU incorporation from the two optic lobes and thoracic segments from 10 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.01, Student's two-tailed t-test). (J) Comparison of the average fluorescence intensity of anti-H3S10ph antibody labeling from the two optic lobes and thoracic segments from 8 brains of wild type and <i>Digitor</i> mutant larvae with S.D., respectively. The difference was statistically significant (P value < 0.001, Student's two-tailed t-test).</p> 2016-11-18 17:59:48 ZNF Drosophila Development P-element insertion paraquat-induced oxidative stress γ H 2Av spindle matrix protein Skeletor yeast two-hybrid screen Multiple Roles ASCIZ spindle region ATMIN pupal development disc eversion pupal lethality stress test paradigm DNA double-strand breaks mitotic localization puff chromosomal regions mitotic progression DNA damage response spindle matrix mCitrine-labeled Digitor brain size Phenotypic analysis