Confocal time-lapse analysis of 3xHA-Digitor-mCitrine during mitosis in a syncytial <i>Drosophila</i> embryo. Saheli Sengupta Uttama Rath Changfu Yao Michael Zavortink Chao Wang Jack Girton Kristen M. Johansen Jørgen Johansen 10.1371/journal.pone.0166829.g006 https://plos.figshare.com/articles/figure/Confocal_time-lapse_analysis_of_3xHA-Digitor-mCitrine_during_mitosis_in_a_syncytial_i_Drosophila_i_embryo_/4243973 <p>The figure shows the relative dynamics of the 3xHA-Digitor-mCitrine construct (in green) and tubulin-mCherry (in red) at interphase and metaphase, respectively. Arrows indicate the localization of the 3xHA-Digitor-mCitrine construct in the spindle region after NEB and microtubule spindle formation at metaphase.</p> 2016-11-18 17:59:48 ZNF Drosophila Development P-element insertion paraquat-induced oxidative stress γ H 2Av spindle matrix protein Skeletor yeast two-hybrid screen Multiple Roles ASCIZ spindle region ATMIN pupal development disc eversion pupal lethality stress test paradigm DNA double-strand breaks mitotic localization puff chromosomal regions mitotic progression DNA damage response spindle matrix mCitrine-labeled Digitor brain size Phenotypic analysis