TY - DATA T1 - Transgenic expression of 3xHA-Digitor-mCitrine in salivary gland cells. PY - 2016/11/18 AU - Saheli Sengupta AU - Uttama Rath AU - Changfu Yao AU - Michael Zavortink AU - Chao Wang AU - Jack Girton AU - Kristen M. Johansen AU - Jørgen Johansen UR - https://plos.figshare.com/articles/figure/Transgenic_expression_of_3xHA-Digitor-mCitrine_in_salivary_gland_cells_/4243967 DO - 10.1371/journal.pone.0166829.g004 L4 - https://ndownloader.figshare.com/files/6920663 KW - ZNF KW - Drosophila Development KW - P-element insertion KW - paraquat-induced oxidative stress KW - γ H 2Av KW - spindle matrix protein Skeletor KW - yeast two-hybrid screen KW - Multiple Roles KW - ASCIZ KW - spindle region KW - ATMIN KW - pupal development KW - disc eversion KW - pupal lethality KW - stress test paradigm KW - DNA double-strand breaks KW - mitotic localization KW - puff chromosomal regions KW - mitotic progression KW - DNA damage response KW - spindle matrix KW - mCitrine-labeled Digitor KW - brain size KW - Phenotypic analysis N2 - (A) Diagram of 3xHA-Digitor-mCitrine. (B) Polytene squash preparation labeled with anti-HA antibody to detect the 3xHA-Digitor-mCitrine construct (in green) and with Hoechst to label DNA (in blue/grey). In the composite image (comp) examples of developmental puff regions with higher expression of 3xHA-Digitor-mCitrine are indicated by arrowheads. (C) Polytene squash preparation labeled with anti-HA antibody to detect the 3xHA-Digitor-mCitrine construct (in green) and with anti-Skeletor antibody (in red). In the composite image (comp) co-localization between 3xHA-Digitor-mCitrine and Skeletor is indicated by yellow/orange colors. Examples of developmental puff regions positive for 3xHA-Digitor-mCitrine but not for Skeletor are indicated by arrowheads. The relatively low exposure of anti-HA antibody-labeling of 3xHA-Digitor-mCitrine in (B) shows the prominent labeling of the developmental puffs whereas the longer exposure in (C) highlights the labeling of the weaker labeled interband regions. ER -