10.6084/m9.figshare.4212924.v1
Chen H.
Chen
H.
Cao J.
Cao
J.
Zhou C.
Zhou
C.
Liu H.
Liu
H.
Zhang X.
Zhang
X.
Zhou T.
Zhou
T.
Supplementary Material for: Biofilm Formation Restrained by Subinhibitory Concentrations of Tigecyclin in Acinetobacter baumannii Is Associated with Downregulation of Efflux Pumps
Karger Publishers
2016
Acinetobacter baumannii
Tigecycline
Biofilm
Efflux pump
2016-11-07 15:14:08
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Biofilm_Formation_Restrained_by_Subinhibitory_Concentrations_of_Tigecyclin_in_Acinetobacter_baumannii_Is_Associated_with_Downregulation_of_Efflux_Pumps/4212924
<i>Background:</i> Tigecycline, one of the few therapeutic options against multidrug-resistant <i>Acinetobacter baumannii,</i> reaches subinhibitory serum concentrations only with cautious clinical dosing and pharmacokinetics. Subinhibitory concentrations of tigecycline might induce an <i>A. baumannii</i> biofilm. <i>Methods:</i> Biofilm formation was assessed via the crystal violet staining method. We further analyzed the main biofilm components with NaIO4, proteinase K, and DNase. Real-time RT-PCR was applied for quantitative detection of biofilm potential-associated genes. <i>Results:</i> In this study, <i>A. baumannii </i>proved to be a strong biofilm producer, and we found that proteins and extracellular DNA are crucial components of the <i>A. baumannii </i>biofilm. Quantitative real-time RT-PCR revealed positive correlations between biofilm formation restrained by subinhibitory concentrations of tigecycline and the expression of biofilm potential-associated genes, especially the <i>AdeFGH</i> efflux pump gene. <i>Conclusion:</i> Our results suggest that downregulation of efflux pumps, especially the <i>AdeFGH</i> efflux pump, is probably responsible for the decline in biofilm formation in <i>A. baumannii</i> treated with subinhibitory concentrations of tigecyclin.