TY - DATA T1 - Histological analysis of TAT-MafA-treated pancreata in neonate pups. PY - 2011/08/04 AU - Nancy Vargas AU - Silvia Álvarez-Cubela AU - Jaime A. Giraldo AU - Margarita Nieto AU - Nicholas M. Fort AU - Sirlene Cechin AU - Enrique García AU - Pedro Espino-Grosso AU - Christopher A. Fraker AU - Camillo Ricordi AU - Luca Inverardi AU - Ricardo L. Pastori AU - Juan Domínguez-Bendala UR - https://plos.figshare.com/articles/figure/_Histological_analysis_of_TAT_MafA_treated_pancreata_in_neonate_pups_/419127 DO - 10.1371/journal.pone.0022364.g005 L4 - https://ndownloader.figshare.com/files/748768 KW - tat-mafa-treated KW - pancreata KW - neonate N2 - (A) Representative section of a control pancreas (in utero injection: vehicle), showing a disorganized pattern of β cell expression (red) in islets. (B) Representative section of a pancreas of a neonate pup treated in utero with TAT-MafA. Islets are larger, rounder and more organized than in controls. Size bars: 500 µm. (C–D) High magnification confocal microphotograph of control neonatal (C) and TAT-MafA-treated (D) pancreata. While the former exhibits small islets in the process of coalescence, islets from the latter are already larger in size. Prohormone convertase (PC) 1/3 staining (green) was similar in both samples despite a trend in favor of the experimental group by qRT-PCR (fig. 4). Glucagon staining (purple) was stronger in (D) at the same exposure. Size bars: 75 µm. (E) Glut-2 staining (green) is not evident in control samples, but can be observed in TAT-MafA samples (F). Size bars: 75 µm. ER -