%0 Figure %A Vargas, Nancy %A Álvarez-Cubela, Silvia %A A. Giraldo, Jaime %A Nieto, Margarita %A M. Fort, Nicholas %A Cechin, Sirlene %A García, Enrique %A Espino-Grosso, Pedro %A A. Fraker, Christopher %A Ricordi, Camillo %A Inverardi, Luca %A L. Pastori, Ricardo %A Domínguez-Bendala, Juan %D 2011 %T Histological analysis of TAT-MafA-treated pancreata in neonate pups. %U https://plos.figshare.com/articles/figure/_Histological_analysis_of_TAT_MafA_treated_pancreata_in_neonate_pups_/419127 %R 10.1371/journal.pone.0022364.g005 %2 https://ndownloader.figshare.com/files/748768 %K tat-mafa-treated %K pancreata %K neonate %X

(A) Representative section of a control pancreas (in utero injection: vehicle), showing a disorganized pattern of β cell expression (red) in islets. (B) Representative section of a pancreas of a neonate pup treated in utero with TAT-MafA. Islets are larger, rounder and more organized than in controls. Size bars: 500 µm. (C–D) High magnification confocal microphotograph of control neonatal (C) and TAT-MafA-treated (D) pancreata. While the former exhibits small islets in the process of coalescence, islets from the latter are already larger in size. Prohormone convertase (PC) 1/3 staining (green) was similar in both samples despite a trend in favor of the experimental group by qRT-PCR (fig. 4). Glucagon staining (purple) was stronger in (D) at the same exposure. Size bars: 75 µm. (E) Glut-2 staining (green) is not evident in control samples, but can be observed in TAT-MafA samples (F). Size bars: 75 µm.

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