%0 Figure %A R. Lovewell, Rustin %A M. Collins, Ryan %A L. Acker, Julie %A A. O'Toole, George %A J. Wargo, Matthew %A Berwin, Brent %D 2011 %T Successive loss of flagellar functionality enables stepwise increases in phagocytic resistance. %U https://plos.figshare.com/articles/figure/_Successive_loss_of_flagellar_functionality_enables_stepwise_increases_in_phagocytic_resistance_/406587 %R 10.1371/journal.ppat.1002253.g007 %2 https://ndownloader.figshare.com/files/6779238 %K phagocytic cells %K flagellar motility result %K bacterium Pseudomonas aeruginosa %K Bacterial Flagellar Torsion Confers Progressive Phagocytic Evasion Phagocytosis %K phagocytic evasion %K flagellar motility %K phagocytosi %K non-opsonized phagocytic recognition %K gamma proteobacterial group %X

(A) FACS analysis and (B) gentamicin protection assay of BMDCs co-incubated with PA14 WT, motAB, motCD, or motABmotCD. (C) FACS analysis of cytochalasin D treated BMDCs co-incubated with GFP-expressing PA14 WT, flgK, motAB, motCD, or motABmotCD. (D) Adherent macrophage assay of cells co-incubated with PA14 WT, motAB, motCD, or motABmotCD bacteria at 4°C, warmed to 37 °C, or treated with gentamicin after warming to 37 °C. (E) Gentamicin protection assay of murine peritoneal macrophages co-incubated as indicated with P. aeruginosa PA14 WT or V. cholerae O395 WT in 70 mM, 18 mM, 9 mM, or 4.5 mM NaCl buffers, or in 15 mM NaCl buffer with 135 mM choline chloride. Recovered CFUs are normalized against recovery in HBSS (138 mM NaCl). N≥5, *p<0.05.

%I PLOS Pathogens