TY - DATA T1 - Supplementary Material for: Histaminergic H3-Heteroreceptors as a Potential Mediator of Betahistine-Induced Increase in Cochlear Blood Flow PY - 2016/10/25 AU - Bertlich M. AU - Ihler F. AU - Freytag S. AU - Weiss B.G. AU - Strupp M. AU - Canis M. UR - https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Histaminergic_H3-Heteroreceptors_as_a_Potential_Mediator_of_Betahistine-Induced_Increase_in_Cochlear_Blood_Flow/4056555 DO - 10.6084/m9.figshare.4056555.v1 L4 - https://ndownloader.figshare.com/files/6568770 L4 - https://ndownloader.figshare.com/files/6568773 L4 - https://ndownloader.figshare.com/files/6568776 KW - Betahistine KW - H3-receptors KW - H1-receptors KW - α2-receptors KW - Ménière’s disease KW - Cochlea KW - Microcirculation KW - Fluorescence microscopy N2 - Objective: Betahistine is a histamine-like drug that is considered beneficial in Ménière's disease by increasing cochlear blood flow. Acting as an agonist at the histamine H1-receptor and as an inverse agonist at the H3-receptor, these receptors as well as the adrenergic α2-receptor were investigated for betahistine effects on cochlear blood flow. Materials and Methods: A total of 54 Dunkin-Hartley guinea pigs were randomly assigned to one of nine groups treated with a selection of H1-, H3- or α2-selective agonists and antagonists together with betahistine. Cochlear blood flow and mean arterial pressure were recorded for 3 min before and 15 min after infusion. Results: Blockage of the H3- or α2-receptors caused a suppression of betahistine-mediated typical changes in cochlear blood flow or blood pressure. Activation of H3-receptors caused a drop in cochlear blood flow and blood pressure. H1-receptors showed no involvement in betahistine-mediated changes of cochlear blood flow. Conclusion: Betahistine most likely affects cochlear blood flow through histaminergic H3-heteroreceptors. ER -