%0 Figure %A Feldbauer, Katrin %A Schlegel, Jan %A Weissbecker, Juliane %A Sauer, Frank %A Wood, Phillip G. %A Bamberg, Ernst %A Terpitz, Ulrich %D 2016 %T Patch-clamp investigation of the optochemokine tandem in cell attached configuration at 34°C. %U https://plos.figshare.com/articles/figure/Patch-clamp_investigation_of_the_optochemokine_tandem_in_cell_attached_configuration_at_34_C_/4053528 %R 10.1371/journal.pone.0165344.g004 %2 https://ndownloader.figshare.com/files/6525294 %K tandem endosomes %K cytosol %K light-induced release %K dyes rhod 2 %K shuttle protein %K plasma membrane %K Optochemokine Tandem %K GPCR %K rhod 2-AM %K rhodopsin tandem %K internalization kinetics %K intracellular areas %K chemokine receptor CXCR 4 %K CatCh signal %K confocal laser scanning microscopy %K patch-clamp measurements %K optochemokine tandem %K tCXCR %K SDF %X

The pipette solution was supplemented with 50 nM SDF1α. a. Typical current trace recorded at an applied membrane potential of -100 mV. Light-dependent signal of tCXCR4/CatCh directly after the sealing process and 30 min later. During that time the cell was illuminated every two minutes for 100 ms. b. Time course of the relative tCXCR4/CatCh current in presence of SDF1α (square, 5 cells) or SDF1α and the inhibitor AMD3100 (triangle, 3 cells), and CatCh in presence of SDF1α (circle, 4 cells). Mean values are given, bars represent the standard error.

%I PLOS ONE