10.1371/journal.pone.0165344.g004
Katrin Feldbauer
Katrin
Feldbauer
Jan Schlegel
Jan
Schlegel
Juliane Weissbecker
Juliane
Weissbecker
Frank Sauer
Frank
Sauer
Phillip G. Wood
Phillip G.
Wood
Ernst Bamberg
Ernst
Bamberg
Ulrich Terpitz
Ulrich
Terpitz
Patch-clamp investigation of the optochemokine tandem in cell attached configuration at 34°C.
Public Library of Science
2016
tandem endosomes
cytosol
light-induced release
dyes rhod 2
shuttle protein
plasma membrane
Optochemokine Tandem
GPCR
rhod 2-AM
rhodopsin tandem
internalization kinetics
intracellular areas
chemokine receptor CXCR 4
CatCh signal
confocal laser scanning microscopy
patch-clamp measurements
optochemokine tandem
tCXCR
SDF
2016-10-21 17:37:04
Figure
https://plos.figshare.com/articles/figure/Patch-clamp_investigation_of_the_optochemokine_tandem_in_cell_attached_configuration_at_34_C_/4053528
<p>The pipette solution was supplemented with 50 nM SDF1α. <b>a.</b> Typical current trace recorded at an applied membrane potential of -100 mV. Light-dependent signal of tCXCR4/CatCh directly after the sealing process and 30 min later. During that time the cell was illuminated every two minutes for 100 ms. <b>b.</b> Time course of the relative tCXCR4/CatCh current in presence of SDF1α (square, 5 cells) or SDF1α and the inhibitor AMD3100 (triangle, 3 cells), and CatCh in presence of SDF1α (circle, 4 cells). Mean values are given, bars represent the standard error.</p>