10.1371/journal.pone.0165344.g004 Katrin Feldbauer Katrin Feldbauer Jan Schlegel Jan Schlegel Juliane Weissbecker Juliane Weissbecker Frank Sauer Frank Sauer Phillip G. Wood Phillip G. Wood Ernst Bamberg Ernst Bamberg Ulrich Terpitz Ulrich Terpitz Patch-clamp investigation of the optochemokine tandem in cell attached configuration at 34°C. Public Library of Science 2016 tandem endosomes cytosol light-induced release dyes rhod 2 shuttle protein plasma membrane Optochemokine Tandem GPCR rhod 2-AM rhodopsin tandem internalization kinetics intracellular areas chemokine receptor CXCR 4 CatCh signal confocal laser scanning microscopy patch-clamp measurements optochemokine tandem tCXCR SDF 2016-10-21 17:37:04 Figure https://plos.figshare.com/articles/figure/Patch-clamp_investigation_of_the_optochemokine_tandem_in_cell_attached_configuration_at_34_C_/4053528 <p>The pipette solution was supplemented with 50 nM SDF1α. <b>a.</b> Typical current trace recorded at an applied membrane potential of -100 mV. Light-dependent signal of tCXCR4/CatCh directly after the sealing process and 30 min later. During that time the cell was illuminated every two minutes for 100 ms. <b>b.</b> Time course of the relative tCXCR4/CatCh current in presence of SDF1α (square, 5 cells) or SDF1α and the inhibitor AMD3100 (triangle, 3 cells), and CatCh in presence of SDF1α (circle, 4 cells). Mean values are given, bars represent the standard error.</p>