S. Istivan, Taghrid Pirogova, Elena Gan, Emily M. Almansour, Nahlah J. Coloe, Peter Cosic, Irena Cellular viability of mammalian cell lines treated with a single dose of different concentrations of the RRM peptides up to 72 h after treatment. <p><b>A.</b> CHO, <b>B.</b> B16F0 and <b>C.</b> COLO16 cells. Cell cultures (1×10<sup>5</sup>) were incubated for 4 h, 8 h, 16 h, 24 h, 48 h and 72 h with (50 ng/ml, 100 ng/ml, 200 ng/ml, 400 ng/ml and 800 ng/ml) of RRM-MV and 400 ng/ml of RRM-C. A blank (no treatment control) and a positive control (treated with 60% DMSO) were included in all assays. OD was measured at 570 nm after addition of the Prestoblue™ reagent and incubation for 30 min. Cell viability was calculated and is shown relative to that of untreated (blank) sample (set to 100%). Each bar represents mean ± standard errors of 3 separate experiments in triplicate. Data values that are significantly altered (ANOVA and Dunnett's post hoc analysis) when compared to the untreated cells are indicated by the star symbol (<i>p</i><0.05).</p> viability;mammalian;lines;treated;concentrations;rrm;peptides;72 2013-02-20
    https://plos.figshare.com/articles/figure/_Cellular_viability_of_mammalian_cell_lines_treated_with_a_single_dose_of_different_concentrations_of_the_RRM_peptides_up_to_72_h_after_treatment_/404699
10.1371/journal.pone.0024809.g006