TY - DATA T1 - Zrf1 Controls Mesoderm Lineage Genes and Cardiomyocyte Differentiation PY - 2016/10/18 AU - Aysegül Kaymak AU - Holger Richly UR - https://tandf.figshare.com/articles/dataset/Zrf1_Controls_Mesoderm_Lineage_Genes_and_Cardiomyocyte_Differentiation/4039980 DO - 10.6084/m9.figshare.4039980.v1 L4 - https://ndownloader.figshare.com/files/6504153 L4 - https://ndownloader.figshare.com/files/6504156 L4 - https://ndownloader.figshare.com/files/6504159 L4 - https://ndownloader.figshare.com/files/6504162 L4 - https://ndownloader.figshare.com/files/6504165 L4 - https://ndownloader.figshare.com/files/6504168 L4 - https://ndownloader.figshare.com/files/6504171 L4 - https://ndownloader.figshare.com/files/6504174 KW - heart tissue-specific genes KW - generation KW - Zrf 1 Controls Mesoderm Lineage Genes KW - TSS KW - P 19 systems KW - transcriptional activator Zrf 1 KW - P 19 cells KW - Zrf 1 KW - expression N2 - In the present study we addressed the function of the transcriptional activator Zrf1 in the generation of the three germ layers during in vitro development. Currently, Zrf1 is rather regarded as a factor that drives the expression of neuronal genes. Here, we have employed mouse embryonic stem cells and P19 cells to understand the role of Zrf1 in the generation of mesoderm-derived tissues like adipocytes, cartilage and heart. Our data shows that Zrf1 is essential for the transcriptional activation of genes that give rise to mesoderm and in particular heart development. In both, the mESC and P19 systems, we provide evidence that Zrf1 contributes to the generation of functional cardiomyocytes. We further demonstrate that Zrf1 binds to the transcription start sites (TSSs) of heart tissue-specific genes from the first and second heart field where it drives their temporal expression during differentiation. Taken together, we have identified Zrf1 as a novel regulator of the mesodermal lineage that might facilitate spatiotemporal expression of genes. ER -