Validation of the RNA-Seq results using quantitative real-time PCR.
Jian Huang
Lei Zhao
Ping Yang
Zhen Chen
Ni Tang
Xiong Z. Ruan
Yaxi Chen
10.1371/journal.pone.0164787.g006
https://plos.figshare.com/articles/figure/Validation_of_the_RNA-Seq_results_using_quantitative_real-time_PCR_/4039317
<p>The values are presented as the means±SD (n = 4). Comparisons among groups were determined with independent-sample t-test using the SPSS software (Version 11.5). Significant differences are considered at *P<0.05, ** p < 0.001 and *** p < 0.0001 versus control group.β-actin was used as the reference gene.</p>
2016-10-17 17:33:25
CD 36
gene ontology
CD 36 overexpression
CD 36OE cells
HepG 2.2.15 Cells
CD 36 Overexpression
HBV life cycle
HBV gene expression
HepG 2.2.15-CD overexpression
DEG
gene expression
Recent studies
HCV
Hepatitis B Virus Life Cycle Hepatitis B virus
HBV replication
qPCR analysis
genome-wide sequencing
hepatocyte-specific DNA virus
Regulatory Role
RNA-Seq data
hepatitis C virus
141 differentially
HepG 2.2.15 cells
immunodeficiency virus
CD 36 expression
HepG 2.2.15-vector cells
acid translocase CD 36
HIV
KEGG pathway analysis
Genome-Wide Transcriptome Analysis
62 downregulated genes
RNA Sequencing
79 upregulated genes
transcriptomic profile