Validation of the RNA-Seq results using quantitative real-time PCR. Jian Huang Lei Zhao Ping Yang Zhen Chen Ni Tang Xiong Z. Ruan Yaxi Chen 10.1371/journal.pone.0164787.g006 https://plos.figshare.com/articles/figure/Validation_of_the_RNA-Seq_results_using_quantitative_real-time_PCR_/4039317 <p>The values are presented as the means±SD (n = 4). Comparisons among groups were determined with independent-sample t-test using the SPSS software (Version 11.5). Significant differences are considered at *P<0.05, ** p < 0.001 and *** p < 0.0001 versus control group.β-actin was used as the reference gene.</p> 2016-10-17 17:33:25 CD 36 gene ontology CD 36 overexpression CD 36OE cells HepG 2.2.15 Cells CD 36 Overexpression HBV life cycle HBV gene expression HepG 2.2.15-CD overexpression DEG gene expression Recent studies HCV Hepatitis B Virus Life Cycle Hepatitis B virus HBV replication qPCR analysis genome-wide sequencing hepatocyte-specific DNA virus Regulatory Role RNA-Seq data hepatitis C virus 141 differentially HepG 2.2.15 cells immunodeficiency virus CD 36 expression HepG 2.2.15-vector cells acid translocase CD 36 HIV KEGG pathway analysis Genome-Wide Transcriptome Analysis 62 downregulated genes RNA Sequencing 79 upregulated genes transcriptomic profile