10.1371/journal.pone.0164787.g006 Jian Huang Jian Huang Lei Zhao Lei Zhao Ping Yang Ping Yang Zhen Chen Zhen Chen Ni Tang Ni Tang Xiong Z. Ruan Xiong Z. Ruan Yaxi Chen Yaxi Chen Validation of the RNA-Seq results using quantitative real-time PCR. Public Library of Science 2016 CD 36 gene ontology CD 36 overexpression CD 36OE cells HepG 2.2.15 Cells CD 36 Overexpression HBV life cycle HBV gene expression HepG 2.2.15-CD overexpression DEG gene expression Recent studies HCV Hepatitis B Virus Life Cycle Hepatitis B virus HBV replication qPCR analysis genome-wide sequencing hepatocyte-specific DNA virus Regulatory Role RNA-Seq data hepatitis C virus 141 differentially HepG 2.2.15 cells immunodeficiency virus CD 36 expression HepG 2.2.15-vector cells acid translocase CD 36 HIV KEGG pathway analysis Genome-Wide Transcriptome Analysis 62 downregulated genes RNA Sequencing 79 upregulated genes transcriptomic profile 2016-10-17 17:33:25 Figure https://plos.figshare.com/articles/figure/Validation_of_the_RNA-Seq_results_using_quantitative_real-time_PCR_/4039317 <p>The values are presented as the means±SD (n = 4). Comparisons among groups were determined with independent-sample t-test using the SPSS software (Version 11.5). Significant differences are considered at *P<0.05, ** p < 0.001 and *** p < 0.0001 versus control group.β-actin was used as the reference gene.</p>