10.6084/m9.figshare.4028886.v1
Agnik Dasgupta
Agnik
Dasgupta
Jeffrey D. Amack
Jeffrey D.
Amack
Time-lapse imgaing of KV development from Cilia in vertebrate left–right patterning
The Royal Society
2016
left–right asymmetry
birth defects
motile cilia
mechanosensory cilia
fluid flow dynamics
2016-10-14 09:24:49
Media
https://rs.figshare.com/articles/media/Time-lapse_imgaing_of_KV_development_from_Cilia_in_vertebrate_left_right_patterning/4028886
Time-lapse video microscopy during DFC to KV transition. Confocal time-lapse video imaging of mosaic labeled DFC/KV cells from Tg(ubi:Zebrabow);Tg(sox17:CreERT2) double transgenic embryos. Cell behaviors were monitored for 60 min. starting at 90% epiboly stage. This developmental window includes migration and rearrangement of cells within DFC cluster and consequent KV lumen formation. Cells do not rearrange stochastically, but rather prefer to maintain their positional information during DFC to KV morphogenesis.