10.1371/journal.pone.0153621.g005
Sandra M. Davern
Sandra M.
Davern
Timothy E. McKnight
Timothy E.
McKnight
Robert F. Standaert
Robert F.
Standaert
Jennifer L. Morrell-Falvey
Jennifer L.
Morrell-Falvey
Elena D. Shpak
Elena
D. Shpak
Udaya C. Kalluri
Udaya
C. Kalluri
Joanna Jelenska
Joanna
Jelenska
Jean T. Greenberg
Jean
T. Greenberg
Saed Mirzadeh
Saed
Mirzadeh
Carbon nanofiber arrays deliver fluorescent OG-CmPP16-1 into a <i>Populus</i> leaf.
Public Library of Science
2016
tissue integrity
hydrogen peroxide
Populus leaf tissue
picomole quantities
tree species
sub-microliter quantities
Novel Tool
carbon nanofiber
bioenergy applications
microdelivery method
Populus leaf cells
wound response
leaf surface
carbon Nanofiber Arrays
radiolabeled molecules
plant regions
VACNF arrays
2016-04-27 13:59:15
Figure
https://plos.figshare.com/articles/figure/Carbon_nanofiber_arrays_deliver_fluorescent_OG-CmPP16-1_into_a_i_Populus_i_leaf_/4020924
<p>(a) Epifluorescence micrograph of the impaled region. The outline of the VACNF chip (ca. 2 × 2 mm) is indicated by the large dashed box. (b) Close-up view of a leaf vein junction showing carbon nanofiber spikes resident in the leaf. Arrows indicate clusters of fibers, in which individual fibers are visible as small black dots. (c) Composite epifluorescence image showing bidirectional movement of the labeled protein from the site of impalement with the VACNF array. The images were recorded 5–10 min after introduction of OG-CmPP16-1. Results shown are representative of 5 separate experiments (one plant per experiment). (d) Accumulation of fluorescence at the hydathodes was observed in 2 of 5 experiments 30 min after VACNF delivery. The black square indicates the location of the VACNF chip.</p>