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3h1_vs_WT_nuclei

dataset
posted on 04.06.2019 by Célia Baroux, Benoit Mermaz
Datasets for comparison of chromatin organisation in nuclei from wild-type Arabidopsis plants (Col, WT) or linker histone triple mutants (3h1) plants (~2 weeks old seedlings).
1) Images of isolated leaf nuclei stained for DNA (PI) or histone modifications as indicated in their label (lif files, readable by Fiji)
2)TEM images (regions of interest) as used for chromatin density analyses (303=3h1, 277=wt, 276=3h1complemented) (Zip file, TIF images)
3) FRAP series on H2B-RFP labelled nuclei used for mobility analyses (.lif file readable by Fiji/Bioformat), raw data (no crop, no registration). Datasheet, xls file with raw, normalised, relative recovery data and final graphs

Dataset related to: Rutowicz et al , (2019)

Linker histones regulate fine-scale chromatin organization and modulate developmental decisions in Arabidopsis


Funding

SNF 310013A_149974

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