%0 Figure %A Xiao, Yang %A Kwong, Mandy %A Daemen, Anneleen %A Belvin, Marcia %A Liang, Xiaorong %A Hatzivassiliou, Georgia %A O’Brien, Thomas %D 2016 %T Sensitive cell lines show defects in purine and pyrimidine biosynthesis. %U https://plos.figshare.com/articles/figure/Sensitive_cell_lines_show_defects_in_purine_and_pyrimidine_biosynthesis_/3999276 %R 10.1371/journal.pone.0164166.g004 %2 https://ndownloader.figshare.com/files/6446979 %K NAMPT inhibitor GNE -617 %K NAD depletion %K h 1334 cells %K non-small cell lung carcinoma cell lines %K NCI-H %K cell lines %K LKB %K 549 cells %K ROS %K combat oxidative stress %K reactive oxygen species %K tumor growth %K LC-KJ %K NAMPT inhibition %K H 1334 cells %K NADPH %K NMN %K cancer cell lines %K Pentose Phosphate pathway %K stress kinase AMPK %K Variable Nicotinamide adenine dinucleotide %K PPP %K cell lines show %X

A-B) Cells were treated with 0.4 μM GNE-617 and levels of UMP and IMP (A), GMP and UDP (B) were determined as described in Fig 2A (Ave ± SD, n = 5). * indicates a p-value of <0.05, and statistical analysis was performed using a Mann Whitney t-test (two-tailed); “ns” indicates not-statistically significant. C) Level of mono-nucleotides (n = 8), diphosphorylated-nucleotides (n = 7) and triphosphorylated-nucleotides (n = 7) in cells were determined following treatment with 0.4 μM GNE-617 (shown is the average and SD for each group). * indicates a p-value of <0.05, and statistical analysis was performed using a Mann Whitney t-test (two-tailed); “ns” indicates not-statistically significant.

%I PLOS ONE