10.1371/journal.pone.0025676.g003 Arcangela Gabriella Manente Arcangela Gabriella Manente Giulia Pinton Giulia Pinton Daniela Tavian Daniela Tavian Gerardo Lopez-Rodas Gerardo Lopez-Rodas Elisa Brunelli Elisa Brunelli Laura Moro Laura Moro EGR1 induced by EGF is sumoylated and ubiquitinated <i>in vivo.</i> Public Library of Science 2013 induced egf sumoylated ubiquitinated 2013-02-20 14:03:09 Figure https://plos.figshare.com/articles/figure/_EGR1_induced_by_EGF_is_sumoylated_and_ubiquitinated_in_vivo_/397748 <p>(<b>A</b>) ECV304 cells were treated for 1 hour with 10 ng/ml EGF to induce Egr1 expression. Lysates were immunoprecipitated with polyclonal antibody to EGR1 and immunoblotted with anti EGR1 and, after stripping, with anti SUMO-1 antibodies. (<b>B</b>) ECV304 cells were treated 1 hour with 10 ng/ml EGF in the absence or in the presence of 100 µM NEM and lysates obtained were immunoprecipitated with a polyclonal antibodies to EGR1 and immunoblotted with antibodies anti EGR1. The arrows indicate bands corresponding to EGR1 and EGR1 plus SUMO-1. (<b>C</b>) ECV304 cells were treated for 15, 30 or 60 minutes with 10 ng/ml EGF. Lysates were immunoprecipitated with polyclonal antibody to EGR1 and immunoblotted with anti EGR1 and, after stripping, with anti K48 ubiquitin antibodies. (<b>D</b>) ECV304 cells were treated for different times with 10 ng/ml EGF in the absence or in the presence of 100 µM NEM and lysates obtained were resolved by SDS-PAGE and analyzed by Western blot with antibodies against EGR1 and tubulin. All experiments are representative of three independent.</p>