%0 Figure %A Sitapara Leang, Ronika %A Wu, Ting-Ting %A Hwang, Seungmin %A T. Liang, Lidia %A Tong, Leming %A T. Truong, Jennifer %A Sun, Ren %D 2011 %T MHV-68 and KSHV ORF54 diminish IFN-α induced activation of ISRE in a non-dUTPase related manner. %U https://plos.figshare.com/articles/figure/_MHV_68_and_KSHV_ORF54_diminish_IFN_945_induced_activation_of_ISRE_in_a_non_dUTPase_related_manner_/397265 %R 10.1371/journal.ppat.1002292.g001 %2 https://ndownloader.figshare.com/files/726923 %K kshv %K orf54 %K induced %K activation %K isre %K non-dutpase %X

HEK 293T cells were transiently transfected with ISRE_firefly-luciferase, PGK_renilla-luciferase as an internal control, and with expression plasmids for each ORF, control plasmid, or murine cellular dUTPase, then were treated with 3×104 U/mL of human IFN-α. Firefly luciferase values were normalized to renilla luciferase. Percentage of activation is calculated in comparison to control, an unrelated MHV-68 ORF or vector control. Error bars depict one standard error based on four independent trials.

%I PLOS Pathogens