10.1371/journal.pone.0025487.g001
Kenichi Odaka
Kenichi
Odaka
Ichio Aoki
Ichio
Aoki
Junji Moriya
Junji
Moriya
Kaoru Tateno
Kaoru
Tateno
Hiroyuki Tadokoro
Hiroyuki
Tadokoro
Jeff Kershaw
Jeff
Kershaw
Tohru Minamino
Tohru
Minamino
Toshiaki Irie
Toshiaki
Irie
Toshimitsu Fukumura
Toshimitsu
Fukumura
Issei Komuro
Issei
Komuro
Tsuneo Saga
Tsuneo
Saga
Relaxation times and contrast of Mn-labeled MNCs.
Public Library of Science
2013
times
mn-labeled
2013-02-20 13:56:29
Figure
https://plos.figshare.com/articles/figure/_Relaxation_times_and_contrast_of_Mn_labeled_MNCs_/396515
<p>(A) R<sub>1</sub> and R<sub>2</sub> calculated for the <i>in vitro</i> samples are presented. The pelleted 0.1 mM Mn-labeled MNCs showed larger R<sub>1</sub> and R<sub>2</sub> in comparison to the saline control. It was not possible to calculate the R<sub>1</sub> and R<sub>2</sub> values for the MNCs with 0.25 mM Mn-labeling and over (N/A) because the T<sub>2</sub> was too short due to the large susceptibility effect. The R<sub>1</sub>s of the MnCl<sub>2</sub> solutions were approximately linearly correlated with the Mn concentration. The R<sub>1</sub> and R<sub>2</sub> for the supernatant fluid were approximately independent of the initial Mn labeling concentration. (B) T<sub>1</sub>-weighted sagittal images of pelleted MNCs after suspension in 0, 0.1, 0.25, and 0.5 mM Mn solutions. The pelleted MNCs at 0.1 mM MnCl<sub>2</sub>-labeling showed partial signal enhancement in the T<sub>1</sub>-weighted 7 T MRI (arrow). The pelleted MNCs with MnCl<sub>2</sub>-labeling over 0.25 mM lost signal in comparison with the unlabeled (0 mM) control due to the very short T<sub>2</sub> and the T<sub>2</sub>* susceptibility effect at 7 T. In the 0.2 T MRI, the the 0.1, 0.25 and 0.5 mM MnCl<sub>2</sub>-labeled MNCs showed positive contrast, although there may have been some signal loss at the base of the 0.5 mM sample.</p>