10.1371/journal.pone.0151574 Srimathi Bogamuwa Srimathi Bogamuwa Jyan-Chyun Jang Jyan-Chyun Jang Plant Tandem CCCH Zinc Finger Proteins Interact with ABA, Drought, and Stress Response Regulators in Processing-Bodies and Stress Granules Public Library of Science 2016 MARD 1 AtTZF 5 PB plant cells assay GA BiFC plant Tandem CCCH Zinc Finger Proteins Interact ABA signal transduction interaction RD 21A -2-H Stress Response Regulators seed germination responses Arabidopsis thaliana T andem CCCH Z inc F inger proteins yeast two-hybrid screens SG co-localize stress ABA-Regulated Dormancy 1 2016-03-15 00:25:11 Dataset https://plos.figshare.com/articles/dataset/Plant_Tandem_CCCH_Zinc_Finger_Proteins_Interact_with_ABA_Drought_and_Stress_Response_Regulators_in_Processing-Bodies_and_Stress_Granules/3961266 <div><p>Although multiple lines of evidence have indicated that <i>Arabidopsis thaliana</i> <u>T</u>andem CCCH <u>Z</u>inc <u>F</u>inger proteins, AtTZF4, 5 and 6 are involved in ABA, GA and phytochrome mediated seed germination responses, the interacting proteins involved in these processes are unknown. Using yeast two-hybrid screens, we have identified 35 putative AtTZF5 interacting protein partners. Among them, Mediator of ABA-Regulated Dormancy 1 (MARD1) is highly expressed in seeds and involved in ABA signal transduction, while Responsive to Dehydration 21A (RD21A) is a well-documented stress responsive protein. Co-immunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) assays were used to confirm that AtTZF5 can interact with MARD1 and RD21A in plant cells, and the interaction is mediated through TZF motif. In addition, AtTZF4 and 6 could also interact with MARD1 and RD21A in Y-2-H and BiFC assay, respectively. The protein-protein interactions apparently take place in processing bodies (PBs) and stress granules (SGs), because AtTZF5, MARD1 and RD21A could interact and co-localize with each other and they all can co-localize with the same PB and SG markers in plant cells.</p></div>