10.1371/journal.pone.0151914.g005 Raphaël Ioannoni Raphaël Ioannoni Ariane Brault Ariane Brault Simon Labbé Simon Labbé Cuf2 affects RNA polymerase II chromatin occupancy at <i>fzr1</i><sup><i>+</i></sup> and <i>wtf13</i><sup><i>+</i></sup> genes. Public Library of Science 2016 middle-phase genes Mei 4 co-regulate Cuf 2 chromatin immunoprecipitation approach forkhead protein family Mei 4. Bimolecular fluorescence complementation experiments fzr 1 Mei 4 Schizosaccharomyces pombe cuf 2 RNA polymerase II target gene expression meiotic gene expression Mei 4 transcription factor Cuf 2 results Middle-Phase Meiotic Genes 2016-03-17 23:46:20 Figure https://plos.figshare.com/articles/figure/Cuf2_affects_RNA_polymerase_II_chromatin_occupancy_at_i_fzr1_i_sup_i_i_sup_and_i_wtf13_i_sup_i_i_sup_genes_/3956805 <p><i>pat1-114/pat1-114 cuf2</i><sup><i>+</i></sup><i>/cuf2</i><sup><i>+</i></sup> and <i>pat1-114/pat1-114 cuf2Δ/cuf2Δ</i> strains were synchronously induced into meiosis. At the 6-h meiotic time point, chromatin was immunoprecipitated using RNA polymerase II CTD-specific antibodies. Quantification of immunoprecipitated chromatin was performed by qPCR using primers that allowed amplification of several specific DNA regions (A, C, D, ORF A, ORF B, ORF C, and 3’UTR A), which spanned <i>fzr1</i><sup><i>+</i></sup> (panel A), <i>wtf13</i><sup><i>+</i></sup> (panel B), and <i>mei4</i><sup><i>+</i></sup> (panel C) genes. ChIP results are presented as enrichments of specific noncoding and coding regions (<i>fzr1</i><sup><i>+</i></sup>, <i>wtf13</i><sup><i>+</i></sup>, or <i>mei4</i><sup><i>+</i></sup>) relative to a 18S ribosomal DNA coding region. Data were calculated as values of the largest amount of chromatin measured (fold enrichment). Results are shown as the averages ± S.D. of a minimum of three independent experiments. Arrows indicate the direction of transcription. Nucleotide numbers refer to the position relative to the A of the initiator codon of each gene (<i>fzr1</i><sup><i>+</i></sup>, <i>wtf13</i><sup><i>+</i></sup>, or <i>mei4</i><sup><i>+</i></sup>).</p>