10.1371/journal.pone.0151914.g005
Raphaël Ioannoni
Raphaël
Ioannoni
Ariane Brault
Ariane
Brault
Simon Labbé
Simon
Labbé
Cuf2 affects RNA polymerase II chromatin occupancy at <i>fzr1</i><sup><i>+</i></sup> and <i>wtf13</i><sup><i>+</i></sup> genes.
Public Library of Science
2016
middle-phase genes
Mei 4 co-regulate
Cuf 2
chromatin immunoprecipitation approach
forkhead protein family
Mei 4. Bimolecular fluorescence complementation experiments
fzr 1
Mei 4
Schizosaccharomyces pombe cuf 2
RNA polymerase II
target gene expression
meiotic gene expression
Mei 4 transcription factor
Cuf 2 results
Middle-Phase Meiotic Genes
2016-03-17 23:46:20
Figure
https://plos.figshare.com/articles/figure/Cuf2_affects_RNA_polymerase_II_chromatin_occupancy_at_i_fzr1_i_sup_i_i_sup_and_i_wtf13_i_sup_i_i_sup_genes_/3956805
<p><i>pat1-114/pat1-114 cuf2</i><sup><i>+</i></sup><i>/cuf2</i><sup><i>+</i></sup> and <i>pat1-114/pat1-114 cuf2Δ/cuf2Δ</i> strains were synchronously induced into meiosis. At the 6-h meiotic time point, chromatin was immunoprecipitated using RNA polymerase II CTD-specific antibodies. Quantification of immunoprecipitated chromatin was performed by qPCR using primers that allowed amplification of several specific DNA regions (A, C, D, ORF A, ORF B, ORF C, and 3’UTR A), which spanned <i>fzr1</i><sup><i>+</i></sup> (panel A), <i>wtf13</i><sup><i>+</i></sup> (panel B), and <i>mei4</i><sup><i>+</i></sup> (panel C) genes. ChIP results are presented as enrichments of specific noncoding and coding regions (<i>fzr1</i><sup><i>+</i></sup>, <i>wtf13</i><sup><i>+</i></sup>, or <i>mei4</i><sup><i>+</i></sup>) relative to a 18S ribosomal DNA coding region. Data were calculated as values of the largest amount of chromatin measured (fold enrichment). Results are shown as the averages ± S.D. of a minimum of three independent experiments. Arrows indicate the direction of transcription. Nucleotide numbers refer to the position relative to the A of the initiator codon of each gene (<i>fzr1</i><sup><i>+</i></sup>, <i>wtf13</i><sup><i>+</i></sup>, or <i>mei4</i><sup><i>+</i></sup>).</p>