10.1371/journal.pone.0153552.g003 Richard Balint Richard Balint Tristan Lowe Tristan Lowe Tom Shearer Tom Shearer Method adopted for detecting the sample edges. Public Library of Science 2016 tissue medial collateral ligaments acid sample shrinkage scan X-Ray Computed Tomography X-ray iodine potassium iodide solution chemical contrast agents Optimal Contrast Agent 2016-04-14 22:41:18 Figure https://plos.figshare.com/articles/figure/Method_adopted_for_detecting_the_sample_edges_/3948903 <p>First the normalised intensity line profile is filtered with a Gaussian kernel with σ = 4 (red line) in order to reduce noise. The maximum (minimum) intensity gradient was detected and the sample edge (dashed, black line) was identified as the next point towards the sample centre at which the absolute value of the intensity gradient was approximately zero (<0.01).</p>