%0 Figure %A Tajnik, Mojca %A Rogalska, Malgorzata Ewa %A Bussani, Erica %A Barbon, Elena %A Balestra, Dario %A Pinotti, Mirko %A Pagani, Franco %D 2016 %T Identification of SRSF2 as the main splicing factor responsible for FIX exon 5 recognition. %U https://plos.figshare.com/articles/figure/Identification_of_SRSF2_as_the_main_splicing_factor_responsible_for_FIX_exon_5_recognition_/3932919 %R 10.1371/journal.pgen.1006082.g002 %2 https://ndownloader.figshare.com/files/6153030 %K protein function %K coagulation FIX exon 5. %K mutation %K disease-causing %K SRSF 2-dependent enhancement mechanism %K Protein Function Mutations %K therapy %K FIX secretion defect %K U 1snRNP particle %X

(A) Effect of overexpression of a panel of splicing factors on the splicing pattern in the FIX exon 5 wild-type and mutant minigenes (V107V and R116R). Minigenes were transfected in HeLa cells alone (NT) or with the indicated splicing factors and the splicing pattern evaluated by RT-PCR. The identity of the amplified bands with inclusion or exclusion of exon 5 is indicated. (B) Silencing of SRSF2 reduces the percentage of exon 5 inclusion in wild-type minigene. siRNA treated (+) and control HeLa cells (-) were transfected with wt minigenes and the resulting splicing pattern evaluated by RT-PCR. (C) Relative expression level of SRSF2 RNA, analyzed by qRT-PCR in siSRSF2 and control (si Luc) Hela cells. All data in the three panels represent the average of 3 replicates with error bars indicating SD.

%I PLOS Genetics