Quantitative RT-PCR analysis of imprinted gene expression.
Hyun Sik Jang
Yean Ju Hong
Hyun Woo Choi
Hyuk Song
Sung June Byun
Sang Jun Uhm
Han Geuk Seo
Jeong Tae Do
10.1371/journal.pone.0156491.g005
https://plos.figshare.com/articles/figure/Quantitative_RT-PCR_analysis_of_imprinted_gene_expression_/3932901
<p>The expression profiles of paternal and maternal imprinted genes were analyzed by real-time RT-PCR. All data are normalized to <i>ACTB</i> expression and calibrated on the ESCs, whose gene expression was considered 1 for all genes. Error bars represent mean values ± SEM of three independent experiments. Student’s t-test: ***, p<0.001; **, p<0.01; *, p<0.05.</p>
2016-05-27 20:24:32
Parthenogenetic Imprinting Patterns
ESC
pluripotent fusion partner
DNA
pluripotent state
X chromosome reactivation
pluripotency markers Oct 4
Cell Fusion Differentiated
reprograms genomic imprinting patterns