Quantitative RT-PCR analysis of imprinted gene expression. Hyun Sik Jang Yean Ju Hong Hyun Woo Choi Hyuk Song Sung June Byun Sang Jun Uhm Han Geuk Seo Jeong Tae Do 10.1371/journal.pone.0156491.g005 https://plos.figshare.com/articles/figure/Quantitative_RT-PCR_analysis_of_imprinted_gene_expression_/3932901 <p>The expression profiles of paternal and maternal imprinted genes were analyzed by real-time RT-PCR. All data are normalized to <i>ACTB</i> expression and calibrated on the ESCs, whose gene expression was considered 1 for all genes. Error bars represent mean values ± SEM of three independent experiments. Student’s t-test: ***, p<0.001; **, p<0.01; *, p<0.05.</p> 2016-05-27 20:24:32 Parthenogenetic Imprinting Patterns ESC pluripotent fusion partner DNA pluripotent state X chromosome reactivation pluripotency markers Oct 4 Cell Fusion Differentiated reprograms genomic imprinting patterns