10.1371/journal.pone.0026526.g001 Paola Neri Paola Neri Shunji Tokoro Shunji Tokoro Ryo Kobayashi Ryo Kobayashi Tsuyoshi Sugiyama Tsuyoshi Sugiyama Kouji Umeda Kouji Umeda Takeshi Shimizu Takeshi Shimizu Takao Tsuji Takao Tsuji Yoshikatsu Kodama Yoshikatsu Kodama Keiji Oguma Keiji Oguma Hiroshi Mori Hiroshi Mori Western blot analysis and dot blot analysis. Public Library of Science 2013 blot 2013-02-20 12:34:40 Figure https://plos.figshare.com/articles/figure/_Western_blot_analysis_and_dot_blot_analysis_/393008 <p>(A–C) Western blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were subjected to SDS-PAGE at 2.5 µg/lane. (A) CBB staining of SDS-PAGE gel. (B) Anti-Stx-1 IgY and (C) anti-Stx-2 IgY were applied to the membrane at 20 µg/ml. The marks in the figures indicate A subunit, A1 fragment and monomeric B subunit. (D, E) Dot blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were blotted at 4 µg/dot, and Stx-1BH and Stx-2BH at 2 µg/dot. Anti-Stx-1 IgY and anti-Stx-2 IgY were applied to the membrane at 2 µg/ml and 4 µg/ml, respectively.</p>