10.1371/journal.pone.0026526.g001
Paola Neri
Paola
Neri
Shunji Tokoro
Shunji
Tokoro
Ryo Kobayashi
Ryo
Kobayashi
Tsuyoshi Sugiyama
Tsuyoshi
Sugiyama
Kouji Umeda
Kouji
Umeda
Takeshi Shimizu
Takeshi
Shimizu
Takao Tsuji
Takao
Tsuji
Yoshikatsu Kodama
Yoshikatsu
Kodama
Keiji Oguma
Keiji
Oguma
Hiroshi Mori
Hiroshi
Mori
Western blot analysis and dot blot analysis.
Public Library of Science
2013
blot
2013-02-20 12:34:40
Figure
https://plos.figshare.com/articles/figure/_Western_blot_analysis_and_dot_blot_analysis_/393008
<p>(A–C) Western blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were subjected to SDS-PAGE at 2.5 µg/lane. (A) CBB staining of SDS-PAGE gel. (B) Anti-Stx-1 IgY and (C) anti-Stx-2 IgY were applied to the membrane at 20 µg/ml. The marks in the figures indicate A subunit, A1 fragment and monomeric B subunit. (D, E) Dot blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were blotted at 4 µg/dot, and Stx-1BH and Stx-2BH at 2 µg/dot. Anti-Stx-1 IgY and anti-Stx-2 IgY were applied to the membrane at 2 µg/ml and 4 µg/ml, respectively.</p>