TY - DATA T1 - Stabilization of INCENP by Aurora-C phosphorylation. PY - 2016/06/22 AU - Kaori Sasai AU - Hiroshi Katayama AU - David H. Hawke AU - Subrata Sen UR - https://plos.figshare.com/articles/figure/Stabilization_of_INCENP_by_Aurora-C_phosphorylation_/3925614 DO - 10.1371/journal.pone.0157305.g005 L4 - https://ndownloader.figshare.com/files/6143232 KW - Survivin residue Serine 20 KW - Serine 20. Phosphorylation KW - CPC KW - Aurora-B Chromosome Passenger Protein Complex Aurora-C KW - Inner Centromere Protein KW - cancer cells KW - Chromosome Passenger Complex KW - Aurora-C overexpression KW - INCENP overexpressing cells KW - Aurora kinase family KW - INCENP Reveal Shared N2 - (A) Flag-Aurora-C WT, KD, T198A, or T202A mutants were co-transfected with HA-INCENP WT or 3A mutant and GFP empty vector in 293T cells. After 24 h transfection, cells were analyzed. GFP was used as internal control for transfection. Quantification of HA-INCENP protein levels were determined by using the ImageJ software. Relative fold change with normalization by GFP expression is shown. (B) 24 h after transfection, 293T cells were split and grown in culture for additional 24 h. Then cells were treated with 50 μM LLnL for 6 h and analyzed as in A. Experiments (A) and (B) were repeated three times with two biological replicates and figures show the representative results. ER -