10.1371/journal.pone.0158133.g007 Hui-Fang Chang Hui-Fang Chang Ying-Shan Lee Ying-Shan Lee Tang K. Tang Tang K. Tang Ji-Yen Cheng Ji-Yen Cheng The expression of nestin and Sox2 without and with DC pulse stimulation. Public Library of Science 2016 48 h differentiation DC pulse treatment square-wave DC pulses Cortical Neural Precursor Cells DC pulse stimulation EF mNPC progenitor cells NPC cell maintenance medium DC pulses nerve growth factor 2016-06-28 18:36:25 Figure https://plos.figshare.com/articles/figure/The_expression_of_nestin_and_Sox2_without_and_with_DC_pulse_stimulation_/3920127 <p>The percentage of immunostained cells with (A) nestin and (B) Sox2 for mouse neural stem and progenitor cells (mNPCs) cultured with (electrical field [EF]) and without (control [CTL]) square-wave DC pulse stimulation. The <i>P</i> values of Sox2 expression at 3 and 14 DIV for the CTL and EF groups were 0.080 and 0.071, respectively.</p>