Perez-Branguli, Francesc Zagar, Yvrick K. Shanley, Daniel Graef, Isabella A. Chédotal, Alain J. Mitchell, Kevin Sema6A cytoplasmic domain interacts with Abl and Mena. <p>(<b>A</b>) Immunoprecipitations from untreated or PlxnA2-EC-treated COS-7 cells previously transfected with different combinations of myc-Sema6A, Abl-V5 and PlxnA2. Immunoprecipitations (Ip) were performed employing an anti-V5 antibody. Antibodies against V5, myc and PlxnA2 were used in the immunoblots (Ib). (<b>B</b>) Immunoprecipitations from untreated or PlxnA2-EC treated COS-7 cells previously transfected with different combinations of myc-Sema6A, Mena-V5 and PlxnA2. Immunoprecipitations (Ip) were performed employing an anti-myc antibody. Antibodies against V5, myc and PlxnA2 were used in the immunoblots (Ib). (<b>C</b>) and (<b>D</b>) Interactions were quantified by immunoblotting and densitometric analysis. Histograms represent quantification of interaction normalized to the amount of immunoprecipitated protein (Abelson-V5 for Sema6A-Abelson or Sema6A-myc for Sema6A-Mena) from three independent experiments. Students t-test *p<0,05 indicating signifiance. S6A-Abl/S6A-Abl+PlA2 <i>p</i> = 0.013, S6A-Abl-PlA2/S6A-Abl-PlA2+PlA2 <i>p</i> = 0.034. Errors bars represent standard error.</p> cytoplasmic tyrosine kinase;Sema 6A;Semaphorin -6A Regulates Cellular Aggregation;context-dependent interactions;Sema 6A activation;PlxnA 2 2016-07-08
    https://plos.figshare.com/articles/figure/Sema6A_cytoplasmic_domain_interacts_with_Abl_and_Mena_/3906120
10.1371/journal.pone.0158686.g005